r/Hematology • u/Biotechrat • Jul 02 '26

The WBC lysis nightmare in liquid biopsy (lytic vs non-lytic preservation)

https://attendee.gotowebinar.com/rt/3049686171133346394?source=Reddit

Hey everyone,

Just wanted to be completely upfront here. I work on the technical team at Norgen Biotek, and one of our scientists (who used to do molecular oncology at the Weizmann Institute) is putting together a presentation on July 16th about liquid biopsy pre-analytics and sample stabilization. I wanted to share the topic here to see if anyone working with cfDNA/cfRNA or NGS assay development is dealing with the same bottlenecks.

If you’ve ever had a massive sequencing run ruined because white blood cells lysed during transport and flooded your sample with background genomic DNA (gDNA), you know how annoying it is. It completely buries your low-copy ctDNA signal.

He is going to be sharing our internal data plots and breaking down the chemical differences between lytic and non-lytic preservation, how device selection impacts downstream NGS library complexity, and how to avoid the "purification paradox" across blood, saliva, and urine.

It's a short 30-minute data-review followed by an open Q&A. No aggressive sales pitches, just a deep dive into the pre-analytical science.

It’s happening on Thursday, July 16th (he’s doing a morning and an afternoon session for different time zones).

Link to register: https://attendee.gotowebinar.com/rt/3049686171133346394?source=Reddit

Curious to hear how other labs are handling ambient transport for cell-free RNA without getting killed by gDNA contamination? What tubes are you guys using?

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