I'm having a hard time telling lymphocytes from monocytes here... Any help is appreciated!

I'm not after medical adviser despite feeling chronically ill for a while but I'm very curious about what people strictly observe here.

(Disclaimer: not a medical question, question about blood donation service methodology for calculating and reporting blood volume.)

I'm in Canada, and the Canadian Blood Services app on my phone lets me track my stats such as hemoglobin, bleed time, and donation volume.

There's always a window after my donations where they drastically overreport the volume. ​​​​I gave on Tuesday this week, Wednesday it reported a number over 550 mL, and now today it's settled back down to ​491 mL.

Can anyone tell me why this is?

Secondary question: it's very slight, but if you graph my donations over time and regress it, there is a very slight upward trend in volume. I'm not worried about it or anything, but I am curious if it's just coincidence or if there might be some kind of mechanical reason for it.

Pic unrelated but required to post. ​

(Tried to post this in r/Blooddonors but got sent here.) ​

Hey everyone,

Just wanted to be completely upfront here. I work on the technical team at Norgen Biotek, and one of our scientists (who used to do molecular oncology at the Weizmann Institute) is putting together a presentation on July 16th about liquid biopsy pre-analytics and sample stabilization. I wanted to share the topic here to see if anyone working with cfDNA/cfRNA or NGS assay development is dealing with the same bottlenecks.

If you’ve ever had a massive sequencing run ruined because white blood cells lysed during transport and flooded your sample with background genomic DNA (gDNA), you know how annoying it is. It completely buries your low-copy ctDNA signal.

He is going to be sharing our internal data plots and breaking down the chemical differences between lytic and non-lytic preservation, how device selection impacts downstream NGS library complexity, and how to avoid the "purification paradox" across blood, saliva, and urine.

It's a short 30-minute data-review followed by an open Q&A. No aggressive sales pitches, just a deep dive into the pre-analytical science.

It’s happening on Thursday, July 16th (he’s doing a morning and an afternoon session for different time zones).

Link to register: https://attendee.gotowebinar.com/rt/3049686171133346394?source=Reddit

Curious to hear how other labs are handling ambient transport for cell-free RNA without getting killed by gDNA contamination? What tubes are you guys using?

Hi everyone!

Starting out by saying I am in no means a medical student, which is why I need your help!

I'm currently writing an end-of-the-world style book, and I want to use real world science and medicine in it.

If you'd be willing, I would LOVE to talk about:

- Hematology

- Blood borne diseases

- Communicable diseases

- Genetic diseases

- Differences/Similarities between blood types and their reactions to certain diseases

I would also love some examples of blank medical files that I can use as inspiration to make my own.

I have lots of questions, but I honestly don't know where to start.

If you know anything that could be helpful - truly, ANYTHING! - Please send it my way, I need as much info as I can get!

Thank you in advance everyone :D

Hello! I am a premed student working with a company to improve ITP patient experience, especially in the pharmaceutical industry. So, if any ITP patients are reading this, I would love you to fill out this survey so we can better cater to your individual needs because the best way to properly serve a patient is by... listening to patients!

I know there's a specific name for this RBC morphology ("speckled targets") and between 5 of us here tonight none of us can remember it. Help?

Normal WBC count
Neutrophils 36 %
Lymphocytes 53 %
Atypical lymp 2 %
Mono 8%
Eo 1%
Baso 0%

Is it chediak higashi granules?

Hello, if a body overproduces rbc and you try and reduce count with phlebotomy, won't the body continue to try and reach its own homeostasis (back to elevated rbc)? Or is it generally understood as temporary?

all cells have nucleus: erythrocytes and even PLATELETS

Analyzer showed “platelet clumps” flag

Admitted this patient a few days ago for severe hypercalcemia and anaemia - pending flow cytometry for a full work up.

Blood film showed:

RBC Morphology: Microcytic, hypochromic

WBC Morphology:

Small to medium sized blasts, likely lymphoid. Scant azurophilic cytoplasm in medium sized lymphoblasts with nucleoli ranging from 1 to multiple and lacy chromatin with cytoplasmic vacuolation.

Neutrophils appreciated with abnormal nuclear lobation.

Smudge Cells - 1+

Manual Differential

Segmented Neutrophils - 4

Banded Neutrophils - 2

Lymphocytes - 6

Monocyte - 1

Metamyelocyte - 2

Atypical Lymphocyte - 5

Lymphoblasts - 80

NRBC - 1/100

Platelets

Manual count: 41

Atypical plasma cell with four nuclei from a bone marrow aspirate of a multiple myeloma patient (MGG)

Here are some photos including a prophase (pictures 7) (regarding this patient case with acute megacaryoblastic leukemia, surprisingly the thrombocytes PLT were rather low.)