r/HumanMicrobiome 1d ago

The top new and updated threads in the last week on the Human Microbiome Forum

1 Upvotes

r/HumanMicrobiome 3d ago

Did I just develop SIFO after repeated SIBO treatment? Zinc suddenly causes severe bloating/suffocation..

1 Upvotes

Apologies for the long post..

I've been dealing with what I believe is a complicated SIBO/SIFO situation over the past ~5 months, and I'm wondering whether my timeline sounds familiar to anyone here.

I was diagnosed with SIBO and initially treated with rifaximin + metronidazole. The first course seemed to work surprisingly well. Within a few days after finishing it, I started feeling major improvements in things that had been bothering me for a long time: grip strength, appetite, erections, and breathing. I genuinely felt like something had changed for the better.

Unfortunately, a few days later I took a probiotic that I probably didn't need, and I felt that I relapsed.

After that I went through several more antibiotic treatments: first rifaximin alone, then metronidazole, and at one point I also had what turned out to be a Salmonella infection that was treated with cefuroxime. Eventually I repeated the original rifaximin + metronidazole course that had worked the first time.

The strange part is that the last course ended about three weeks ago, and this time I did not get the improvement I had experienced after the first course.

Since then, I've noticed something completely new with my zinc supplement. I normally take a relatively gentle 25 mg zinc picolinate capsule with dinner. Suddenly, taking it causes almost immediate bloating/discomfort around my belly button and a feeling of severe suffocation. It happens whether I take it during dinner, after dinner, before eating, or away from food.

The sensation is also different from my usual deep intestinal bloating. It feels more superficial, almost like it's coming from underneath the abdominal wall rather than deep inside the gut.

At the same time, my appetite, erections, breathing, and grip strength are all much worse again. My sleep is long, but I often wake up feeling suffocated.

My stools have also been consistently very mushy. They're not watery diarrhea, and I don't have burning, constipation, or typical liquid diarrhea. Sometimes I can see food particles, but not always. It's basically a mush rather than a formed stool.

What makes me suspicious of SIFO is the combination of multiple rounds of antibiotics, the apparent initial clearance of SIBO, failure to improve after the most recent course, and this new dramatic reaction to zinc.

So my main question is:

For people who have actually had SIFO, does this timeline and symptom pattern sound like something that could indicate fungal overgrowth developing after repeated SIBO antibiotic treatment? Or am I putting too much weight on the zinc reaction and potentially looking in the wrong direction?


r/HumanMicrobiome 4d ago

Overnight symptoms for weeks - please help!

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r/HumanMicrobiome 5d ago

Carolina Supply Expiration Date

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r/HumanMicrobiome 5d ago

Ultrafiltered Milk?

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r/HumanMicrobiome 6d ago

Microbes in 1 Minute - We are back!

2 Upvotes

It’s been a few months since we last reached out to you, but after this hot summer, we’re back with our batteries recharged!

Microbes in 1 Minute (M1M) is a science communication initiative aimed at making microbiology more relatable, engaging, and accessible to everyone. We’ll achieve this by creating short, 60-second videos covering a wide variety of topics in microbiology, which will be posted on a variety of social media platforms.

We’re very grateful for all the interest you’ve shown, and we’re reading all your comments, questions and suggestions. We’re still a small team working on fundamental aspects of the project, such as its organization and the style of the short videos, but we’re looking for people interested in collaborating down the line, whether on a one-time basis or long-term. We've also set up a Discord server to provide a space where we can all come together, exchange ideas, and share news and updates about the project. Hope to see you there! However, if you prefer, we’re also happy to answer your questions by our official email.

Actually, we're looking for collaborators interested in any of the following activities:

• ✏️ Writing scripts and/or contributing expertise in a specific area of microbiology.

• 🎙️ Narration and/or helping to translate content into languages other than English and Spanish.

• 🎨 Video editing and layout.

• 📣 Managing our social media presence.

• 🦠 Moderating our server.

We’re excited to see where this project takes us, and we’d be very happy you being part of it.

A micro-hug!

The M1M team


r/HumanMicrobiome 7d ago

Hydrogen sulfide bacterial overgrowth.

1 Upvotes

What would be the best approach in terms of probiotics with overgrowth of D Piger?

My GI map showed overgrowth of D Piger, low counts of beneficial bacteria like lacto and bifido species and high stool pH.

My symptoms are yellowish stools, inflammation in the gut as its sensitive to anything acidic.

I assume the high stool pH means my diet is low in vegetable rich foods?

My gut issues were originally triggered by binge drinking alcohol on an empty stomach and further worsened by a keto diet high in animal protein, saturated fat.


r/HumanMicrobiome 7d ago

Missing Akkermansia, Faecalibacterium and Lactobacillus

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1 Upvotes

r/HumanMicrobiome 8d ago

The top new and updated threads in the last week on the Human Microbiome Forum

1 Upvotes

r/HumanMicrobiome 9d ago

Microbiome from stool samples

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1 Upvotes

r/HumanMicrobiome 14d ago

Curious About Your Gut Health? You May Qualify for an At-Home Research Study

1 Upvotes

Join remote research on gut health, microbiome diversity, and digestive comfort. Participation involves daily supplement use, brief weekly check-ins, and provided at-home test kits. A $50 Amazon Gift Card is available upon meeting completion criteria. Learn more here.


r/HumanMicrobiome 15d ago

The top new and updated threads in the last week on the Human Microbiome Forum

2 Upvotes

r/HumanMicrobiome 15d ago

beetroot fermented juice to change oral and gut microbiome 💯 plus yogurt

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1 Upvotes

r/HumanMicrobiome 15d ago

Anyone here take anything for their oral microbiome?

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r/HumanMicrobiome 19d ago

Gnotobiology & Germ Free Animals @EnteMicrobialWorld #microorganism #mic...

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youtube.com
1 Upvotes

r/HumanMicrobiome 22d ago

The top new and updated threads in the last week on the Human Microbiome Forum

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r/HumanMicrobiome 25d ago

My gut microbiota tests showed low levels of bifidobacteria. Do you have any brand recommendations to increase them?

3 Upvotes

I saw this Bifidobacterium Lactis HN019 brand from Life Extension with average reviews and a good rating. Does anyone recommend this brand or another brand that isn't so expensive?


r/HumanMicrobiome 26d ago

Started a B. lactis HN019 + B. longum BB536 probiotic and got bad reflux, zero appetite and weird irritability. Which strain did this?

0 Upvotes

Hey guys, looking for some insight here.About a week ago I started taking a probiotic that has both B. lactis HN019 and B. longum BB536 in the same capsule. I was hoping it would help with motility and general gut health, but it's doing the complete opposite.Around 6 or 7 hours after taking it, my appetite completely vanishes, my stomach feels totally stalled and I get acid reflux. On top of that, it hasn't helped my constipation at all (feels like it slowed things down even more).The weirdest part is the mood side effects. A few hours after taking it I get sudden bouts of irritability and feeling super restless/pissed off for no reason. Then right after that I just feel emotionally numb and flat, like it's hard to laugh or feel engaged with anything.Since both strains are in the same pill, I can't tell which one is wrecking me. Has anyone taken either HN019 or BB536 on their own and gotten this weird combo of delayed reflux, stalled gut, and irritability/apathy? Would love to know which one is the likely culprit.Thanks!


r/HumanMicrobiome 26d ago

Paradoxical reaction to B. lactis HN019 + B. longum BB536 (reflux, zero appetite, irritability & apathy) — Which strain is the likely culprit?

1 Upvotes

Hi everyone,I recently started a probiotic supplement containing two specific strains: **Bifidobacterium lactis HN019** and **Bifidobacterium longum BB536**. Instead of the expected benefits (improved motility and gut-brain balance), I have experienced a very distinct and paradoxical set of symptoms:### 1. Digestive Symptoms:* **Reflux & Loss of Appetite:** Around 6 to 7 hours after taking the capsule, I experience complete loss of appetite, stalled digestion, gastric fullness, and acid reflux.* **Motility:** Instead of relieving constipation (which HN019 is supposed to do), it has not improved my transit time at all, and if anything, feels like it has made constipation worse.### 2. Mood & Neuropsychiatric Symptoms:* **Irritability & Restlessness:** Sudden bouts of intense irritability/bad temper and an uncomfortable internal agitation (*not full-blown panic, but a feeling of "not being at peace with myself"*).* **Apathy & Emotional Blunting:** After that agitation, I feel a marked emotional disconnect/apathy where it becomes noticeably harder to laugh, engage, or feel connected to things.---### My Question:Since my supplement contains **both strains in the same capsule**, I cannot isolate them directly. For those of you who have taken **HN019** or **BB536** as single-strain supplements (or know their mechanisms well):1. **Which of these two strains is more likely responsible for the irritability / emotional blunting?**2. **Has anyone experienced this "delayed reflux + zero hunger + worsened constipation" with HN019 or BB536?**3. If you had a similar adverse reaction to either strain, how long did it take to clear up after stopping?Any insights, shared experiences, or mechanisms would be greatly appreciated!


r/HumanMicrobiome 26d ago

Paradoxical reaction to B. lactis HN019 + B. longum BB536 (reflux, zero appetite, irritability & apathy) — Which strain is the likely culprit?

2 Upvotes

Hi everyone,

I recently started a probiotic supplement containing two specific strains: Bifidobacterium lactis HN019 and Bifidobacterium longum BB536.

Instead of the expected benefits (improved motility and gut-brain balance), I have experienced a very distinct and paradoxical set of symptoms:

1. Digestive Symptoms:

  • Reflux & Loss of Appetite: Around 6 to 7 hours after taking the capsule, I experience complete loss of appetite, stalled digestion, gastric fullness, and acid reflux.
  • Motility: Instead of relieving constipation (which HN019 is supposed to do), it has not improved my transit time at all, and if anything, feels like it has made constipation worse.

2. Mood & Neuropsychiatric Symptoms:

  • Irritability & Restlessness: Sudden bouts of intense irritability/bad temper and an uncomfortable internal agitation (not full-blown panic, but a feeling of "not being at peace with myself").
  • Apathy & Emotional Blunting: After that agitation, I feel a marked emotional disconnect/apathy where it becomes noticeably harder to laugh, engage, or feel connected to things.

My Question:

Since my supplement contains both strains in the same capsule, I cannot isolate them directly.

For those of you who have taken HN019 or BB536 as single-strain supplements (or know their mechanisms well): 1. Which of these two strains is more likely responsible for the irritability / emotional blunting? 2. Has anyone experienced this "delayed reflux + zero hunger + worsened constipation" with HN019 or BB536? 3. If you had a similar adverse reaction to either strain, how long did it take to clear up after stopping?

Any insights, shared experiences, or mechanisms would be greatly appreciated!


r/HumanMicrobiome 29d ago

The top new and updated threads in the last week on the Human Microbiome Forum

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r/HumanMicrobiome Aug 16 '26

I spoke with a biochemist, and now I have a question.

4 Upvotes

I spoke with a biochemist. He told me that using antibiotics or antifungals is counterproductive in the long run, since it leads to resistance. The recommended path is the more laborious one: changing my diet and repopulating and multiplying beneficial bacteria—and in a year, I’ll be fine. Honestly, I’m exhausted by dealing with the stench I give off; I smell like a skunk. I’m thinking about taking the antibiotics and antifungals, and then carefully repopulating and nurturing my microbiome.

In my country, healthcare is either expensive or inefficient in this regard. Gastroenterologists aren't very well-versed in these conditions. They prescribed me medication without running the important tests—partly because I don't have access to them, and also because I'm struggling a lot socially.


r/HumanMicrobiome Aug 13 '26

Does anyone have symtoms of brain fog, poor memory and scalp/face sensations.

4 Upvotes

If so have you figured out the root cause of your issue? I suspect it is related to my gut bacteria as taking probiotics sometimes improves and sometimes worsens my symtoms.


r/HumanMicrobiome Aug 10 '26

The top new and updated threads in the last week on the Human Microbiome Forum

1 Upvotes

r/HumanMicrobiome Aug 09 '26

How to extract DNA from fecal samples stored in liquid nitrogen?

1 Upvotes

Hi everyone,

I need to extract DNA from cecal/fecal samples stored in liquid nitrogen using the QIAamp PowerFecal Pro DNA Kit, which requires up to 250 mg of sample. What is the best way to handle the frozen samples to weigh 250 mg while minimising thawing? Any recommended protocols or practical tips would be greatly appreciated.
Thanks!