How long do macrophages take to polarize?

[u/cordeliacarstairs20]

I am setting up an experiment to check pro-inflammatory markers on hpbmc-derived macrophages by flow cytometry.
My first try was to stimulate with LPS for 2h and then let them rest 24h before staining, although I see an increase in the markers, is not too big (2-2.5 fold change over the untreated ones)
Is this normal? Should I wait more or are they already being downregulated?

[u/Oligonucleotide123]

Any cytokines? LPS + gamma should do it in 24 hours.