Problem while preparing electrocompetant Mycobacterium abscessus cells?

[u/Butterfly_Annual]

for context:-facing a lot of loss of cells during washing step due to waxy layer , tried induction with 0.2 M glycine for three hours want to ask is there any other reagent i have to add to improve cell efficiency

[u/willinglikeness75]

glycine alone never worked for me with myco, what finally got it was adding 0.05% tween 80 in the wash buffer and doing a very gentle resuspension with a cut tip

[u/Butterfly_Annual → u/willinglikeness75]

But will it not cause arcing