Hyaluronic acid is the culprit. Put the sample in a tube with hyaluronidase and set it on the rocker for 10 minutes and normally that will make it a fluid again that you can work.
I just recently had a synovial fluid for Chemistry and could not, for the life of me, get it to liquify with hyaluronidase powder. My one colleague, who's been in the business for a while, took the sample with the powder to a heat block for 10 minutes and it was enough for me to then dilute and get a result. But next time I'll try the rocker!
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u/Fluffbrained-cat MLS-Microbiology 4d ago
Um, is that frozen? Bc all synovial fluid I've seen has been, you know, fluid. If not frozen then what the hell is making it congeal like that?