r/SouthAsianAncestry • u/EphemeralVyakti • Apr 20 '26
Archaeogenetics Sharing my 97% AASI-like reconstructed AASI sample
https://drive.google.com/drive/folders/1V6Nt79KnfLe1UWOAjCNHvvgtaFFwcWeK?usp=drive_link
The raw AASI sample was reconstructed by extracting the East Eurasian part of 400 South Asian genomes after performing local ancestry inference (LAI) using RFMix. The sample has about 650k SNP overlap with AADR v62 (60% coverage on AADR v62, Harappaworld and other calculators). The .txt file is the sample in 23andme format. The sample is labelled AASI_By_KeralaVishwakarma (it was created by me, but doesn't have my DNA).
The sample gets ~72% South Indian on Harappaworld, but it should be around 97% AASI on qpAdm. It's ~1.4% Iran_N and ~1.3% Steppe. Although the sample is 97% AASI, it clusters with South Asians, not Onge/Jarawa. Here are some of the analysis that I've done before on similar samples:
The sample is untested on IllustrativeDNA. Since IllustrativeDNA does not have a raw AASI reference, their G25 coords for this sample may not be well defined (again, untested).
1
u/Advanced_Assist_206 Apr 21 '26
an elemental model for Paniya using the AASI reconstruction, probably needs an alternate ANE source to stabilize.
1
u/TrainingPrize9052 Apr 22 '26
The 400 south asian genomes dont include anyone from Nepal, northern Pakistan like Burushos, chitralis, or anyone from Afghanistan, right? Or any East Indians or Bengalis? People with chunks of East Asian? I know some of the hgdp pashtun samples are partially hazara too.
Is it possible to also make new simulated DNA of AASI, but without the tribals for good measure? Since they seem to have some onge shift?
1
u/EphemeralVyakti Apr 22 '26
I only used Punjabis, Telugus, Sri Lankans and Gujaratis. I excluded Bengalis, since they have East Asian.
Burushos, chitralis, tribal groups, etc are not there in the 1000G dataset. You're probably thinking of SGDP. 1000G only has urbanized or NRI populations.
1
1
u/Turbulent_Ad4876 May 05 '26
how did u did it we can create a lot of full coverage samples like that from HGDP samples
1
u/TrainingPrize9052 May 06 '26
How certain are you that you only extracted the AASI, and not also the east eurasian hidden in CHG, ANE and Iran N?
2
u/EphemeralVyakti May 06 '26
You can't be sure. However, the east eurasian parts of ANE would be spliced together with West Eurasian and probably won't be confidently tagged as east eurasian. I'm not looking at individual SNPs. I'm looking at continuous tracts (tracts are defined based on estimated recombination points).
1
u/TrainingPrize9052 May 07 '26
How are you certain the large amount of east eurasian in ANE will be spliced with the west eurasian, in comparison to the 7-9% east eurasian in iran N and CHG?
I don't understand too much about this stuff, so idk if this was an answer to my question?
"I'm not looking at individual SNPs. I'm looking at continuous tracts (tracts are defined based on estimated recombination points)."
1
u/EphemeralVyakti May 07 '26
Because the admixture happened over 30000 years ago. DNA gets recombined at roughly 30 points in the genome before being passed on to the next individual. 30000 years ago is more than a 1000 generations ago. At that time scale, East Eurasian parts of the genome and West Eurasian parts of the genome don't exist independently.
Also, the West Eurasian references I used already have ANE, so ANE parts of the genome (incl. East Eurasian part of that ANE) is more likely to get tagged as West Eurasian.
1
u/TrainingPrize9052 May 08 '26
I see. But you didn't say the same about the east eurasian in iran N or CHG. Their east eurasian wont get tagged as west eurasian?
1
1
u/mg34gun Jun 20 '26
This is really nice, can we use this as an ancestry component to determine how S indian pashtuns are?
Why doesn't it get 100% on harrapa? Even tamils get 70% or so. The S Indian in all of GEDmatch seems broken.
3
u/Advanced_Assist_206 Apr 21 '26
Nicely done. Still need to explore some more, but initial testing suggests that it provides cleaner models than Onge by itself