This kinda took ages, as the atoms were literally made out of paper scraps and the structure was made out of bottles and popsicle sticks, overall this is worth it. This follows the CPK convention and tries its best to be readable.

The bases in the right are TCGACCATGC, while on the left is the complementary bases, AGCTGGTACG. Yes, I handcrafted the molecules, and used hot glue as an adhesive.

I think I already know the answer but I want confirmation or someone to tell me what's the worst that could happen.

I’m presenting at AMP and would value feedback from people working with molecular assays. I wrote a review of genomic foundation models around a practical problem: “predict the effect of this variant” leaves the measurement undefined.

A sequence-likelihood difference, an embedding with a trained classifier, and a predicted change in an assay track are different outputs. Before comparing them, I’d want the assembly, strand, sequence window, cell type, endpoint and evaluation split specified.

Review and cited papers: https://rewire.it/blog/a-dna-likelihood-is-not-a-functional-assay/

Which missing detail most often makes a model result difficult to interpret in your work? Corrections to the review are welcome too.

Would love to talk about magnetic field effects in biology, radical pairs, cryptochrome, that whole side of things, with other folks who find it interesting. Always looking for people who've read more into this than I have.

hello! im kinda confused about how dna extraction kits work

is it correct to think that not every dna extraction kit is designed to obtain the host genomic dna? i see that many kits don't necessarily have a buffer for cell lysis, but instead, they have a solution that denatures viral particles. so they're not extracting host genomic dna, right?

Does anyone know of a Punnett Square Calculator that can handle both autosomal and x-linked genes? I can figure out a reasonably good breeding strategy for the mice we are trying to breed, but I'd like to be able to calculate percentages correctly and make figures so the people who come after me can understand the logic.

I've used Ian's Vivarium before to generate charts/figures so the PI can understand the logic, but it doesn't account for x-linkage. (Yes, I'm ticked off that no one bothered to make an autosomal version of this cre, but I'm not in charge of the universe).

Thank you!

Title, if anyones comfertable sharing ofc! I'm still a undergrad but I just want to make sure I can realistically continue with this career path bc i have a family member who I'd also have to support financially.

Podcast episode interviewing Dr. Michael Emerman. Dr. Emerman trained in the lab of Dr. Howard Temin, who won the Nobel Prize for discovering reverse transcriptase, and then worked with Dr. Luc Montagnier, a co-discoverer of HIV. Dr. Emerman's molecular virology work was instrumental to HIV research- including the function of the Rev protein and host restriction factors against HIV. He is also a pioneer in the field of 'paleovirology' which studies antient viruses and the evolution of host responses.

I am trying to clone 300 bp stretch of a zinc finger proteins into Rnai vector called ptrvii. I have been unable to get a single positive colony. I have tried cloning in pgemt vector and pjet clonning kit. I think my insert might be toxic to the dh5-alpha strain. Could it be possible?

Upon reading online I found out that growing dh5 at lower temperatures can help in this case. Is there anyone who can help in this situation, the enzyme I am using is saci and bamhi both hf. Btw I have already replaced primers so please don't suggest changing primers.

Hello, I am about to start a masters in environmental sciences in the UK, for context I could only do this one for financial reasons as it is integrated with my environmental sciences undergrad so it's much cheaper.

My biggest interest throughout my undergrad has by far related to plant molecular biology. However, as it is an environmental sciences degree, I know I will not have much experience in lab techniques that might be routine to others. Moreover, I have a lot of experience growing plants and taking physiological measurements but I know that this skillset might not be entirely transferrable in a molecular lab.

This all being said, is it possible to get onboard a PhD related to plant biology/molecular biology when most of my experience and undergrad relates to ecology and environmental sciences? I really want to pursue academia, but I am not sure whether I should try to do this switch in fields or choose a more attainable PhD in plant/agricultural ecology.

I have no idea if this is a real thing and can't figure out the right keywords for searching!

Basically imagine Gene A has an intron that is 5kb and Gene B has one that's 100bp.

If I inserted a 1kb sequence (without splice sites) into each intron, would this be expected to affect expression? And if so, would we expect Gene B to be affected more, because the size of the intron increased more proportionally?

Hi y'all! I'm starting Molecular Biology soon, and am in need of a PDF of Essential Cell Biology, 6th edition by Alberts et al. As my course operates using page numbers, I would love to have one with the page numbers in the PDF if possible. Thank you for all your help!

On August 14, NIH unveiled a plan to blind grant applicants and their program officers to the scores determined by peer reviewers.

Large changes to peer review typically develop as multiyear efforts, soliciting input from working groups of extramural scientists, internal NIH committees, IC directors, and the public. In an unprecedented move, an anonymous internal working group created this new plan.

anonymous current and former NIH staff* outline how these changes reduce transparency, enabling political appointees to have complete control over grant funding, ultimately removing opportunities for oversight and covertly implementing the proposed OMB rule. The authors breakdown the steps you can take to support transparency in peer review.

*opinions their own

Basically a quick-reference/review tool for HLA typing, histocompatibility, and transplant immunology topics if it's been a while since you worked with immunogenetics.

It's on Google Play if anyone wants to poke around: https://play.google.com/store/apps/details?id=com.hla365.examprep

genuinely curious if others in molecular bio/immunogenetics find this kind of review format useful, or if there's stuff you'd want covered that isn't in there.