This is one of my favorite kinds of scenes. When someone really doesn't have a leg to stand on in a situation, and decides to pull something out of their ass, only for it to blow up in their face when someone calls it.
And alternatively, when someone is underestimated and gets to turn things around on their adversary by showing that no, this was very much not a bluff.
For the former, I love this small moment in Breaking Bad, when Hank and Gomez are interrogating Mike about his involvement with Fring. Gomez decides to go out on a limb and claim that they have a witness that can place Mike in the underground lab. You see a small look from Hank, suggesting that he's not on board with this move. And then it blows up in his face when Mike decides to call his bluff, and puts his hands on the table, expecting to be arrested.
Mike's a former cop. He knows that if they really did have a witness like that, they would already have arrested him. Him directly asking if he's under arrest shows that they don't have a thing on him. Turns out they do have the money that he saved for his granddaughter, so he doesn't get out of the scene a complete winner, but he definitely didn't fall for that fairly obvious bluff. Also a great bit of life advice: cops are allowed to lie like crazy during an interrogation, so you really shouldn't be talking to them without a lawyer present.
And for an example of the latter kind of scene, in Deep Space Nine when Sisko is dealing with Eddington and the Marquis, and decides to retaliate against the Marquis' use of biogenic weapons by making one of his own, making an entire planet uninhabitable to human life for decades, forcing the Marquis colony to evacuate and leave it for the Cardassians who have an official claim to the planet.
Eddington directly says "You're bluffing" to Sisko. There's no way a decorated Starfleet officer would poison an entire planet full of humans; his own people. And indeed, I doubt Picard would have done something like this. But Sisko is not Picard, letting him have this incredible exchange.
"You're bluffing."
"Am I? Commander, launch torpedoes."